Overview
Free Protein Standards Selection Magnet
Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.
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Free Protein Standards Temporary Tattoo
Two students celebrated their love of science with Precision Plus Protein Kaleidoscope Ladder tattoos. Read their story and request a free set of matching (temporary) tattoos here.
Precision Plus Protein™ Prestained Standards are available in Dual Color, All Blue, Kaleidoscope™, and Dual Xtra options. All four standards have the same gel migration patterns, with three high-intensity reference bands (25, 50, and 75 kD).
New and Improved Dual Color Standards:
- Brighter for easier identification of target protein
- Sharper for more accurate molecular weight estimation
- Stronger band intensity throughout blot development
The overall brightness of Precision Plus Dual Color Standards has been increased for visual robustness throughout the entire protein electrophoresis and western blotting workflow. Improved Dual Color Standards are now brighter than the competition on both gels and blots.
Dual Xtra Standards
Dual Xtra Standards consist of 12 recombinant proteins between 2 and 250 kD; all others consist of 10 recombinant proteins between 10 and 250 kD (note that the 2 kD band is not visible on fluorescent blots).
Kaleidoscope Standards
Kaleidoscope Standards are a mixture of ten recombinant proteins in four colors (blue, pink, yellow, and green; 10–250 kD), enabling easy gel and blot orientation via the multicolor pattern.
Features and Benefits of Precision Plus Protein Prestained Standards
- MW markers confirmed by mass spectrometry
- Strong linear relationship between markers' log molecular weights and relative migration distances
- Prestained bands for easy visual monitoring of electrophoresis
- Fluorescent dyes for imaging on gels blots
Applications of Precision Plus Protein Prestained Standards
- Accurate molecular weight estimation
- Monitoring of electrophoresis and transfer efficiency
More Information
| Fluorescence Properties of Precision Plus Protein Prestained Standards | |||||
|---|---|---|---|---|---|
| Fluorescent Bands | |||||
| Visible Band Color | Excitation Color | All Blue | Dual Color | Dual Color Extra | Kaleidoscope |
| Blue | 635 nm laser (Red LED) | 10–250 kD | 10–20, 37, 50, 100–250 kD | 5–20, 37, 50, 100–250 kD | 15, 20, 50, 100–250 kD |
| Pink | 532 nm laser (Green LED) | — | 25, 75 kD | 25, 75 kD | 25, 75 kD |
| Green | 635 nm laser (Red LED) | — | — | — | 37 kD |
| Yellow | 488 nm laser (Blue LED) | — | — | — | 10 kD |
See Bulletin 5685 and Bulletin 5723 for more details on the fluorescence properties of Precision Plus Protein Prestained Standards.
Related Technologies
-
Introduction to Polyacrylamide Gels -
Buffer Systems and Gel Chemistries -
Protein Electrophoresis Reagent Selection and Preparation
Related Products
-
Criterion Blotter -
Sample Loading Buffers and Reagents -
Criterion™ Cell -
Running Buffers and Reagents -
PowerPac Universal Power Supply
Specifications
MW size range
2–250 kD
Stain type
Markers consist of highly purified recombinant proteins covalently stained with multicolored dyes
Volume
500 µl (value pack: 2,500 µl)
Total number of applications
50 (value pack: 250 applications)
Loading buffer
30% (w/v) glycerol, 2% SDS,
62.5 mM Tris, pH 6.8, 50 mM DTT,
5 mM EDTA, 0.02% NaN3
Shelf life
Store for 6 months at 4°C or –20°C until expiration date (Kaleidescope: 12 months at –20°C)
Contents
1 x 500 µl tube, instruction sheet, and standards migration sticker
Recommended loading volume
Electrophoresis: 10 μl
Blotting: 7 μl
Ordering
items
Use the filters below to refine results!
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(Discontinued) Precision Plus Protein Dual Color Standards, 500 µl
1610374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
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(Discontinued) Precision Plus Protein™ Dual Color Standards, trial size
1610374S
50 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 5 applications
Image
(Discontinued) Precision Plus Protein™ Dual Color Standards, 2.5 ml
1610394
2.5 ml (5 x 500 µl), mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 250 applications
Image
(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards
1610373
500 µl, mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 50 applications
Image
(Discontinued) Precision Plus Protein™ All Blue Prestained Protein Standards, value pack
1610393
2.5 ml (5 x 500 µl), mixture of ten blue-stained recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 250 applications
Image
(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards
1610375
500 µl, mixture of ten multicolor recombinant proteins (10–250 kD), 50 applications
Image
(Discontinued) Precision Plus Protein™ Kaleidoscope™ Prestained Protein Standards, value pack
1610395
2.5 ml (5 x 500 µl), mixture of ten multicolor recombinant proteins (10–250 kD), 250 applications
Image
(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards
1610377
500 µl, mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 50 applications
Image
(Discontinued) Precision Plus Protein™ Dual Xtra Prestained Protein Standards, value pack
1610397
2.5 ml (5 x 500 µl), mixture of 12 recombinant proteins (2–250 kD), nine blue-stained bands, and three pink reference bands (2, 25, 75 kD), 250 applications
Suggested
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(Discontinued) 2x Laemmli Sample Buffer
1610737
30 ml, premixed protein sample buffer for SDS-PAGE
Image
(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
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(Discontinued) XT Sample Buffer
1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels
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(Discontinued) XT Reducing Agent
1610792
1 ml, 20x reducing agent for use with all Criterion XT gels
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(Discontinued) Tricine Sample Buffer for Protein Gels, 30 ml
1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250
Image
(Discontinued) Precision Plus Protein™ Unstained Protein Standards, Strep-tagged recombinant, 1 ml
1610363
1 ml, unstained mixture of ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands (25, 50, 75 kD), 100 applications
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(Discontinued) Precision Plus Protein™ WesternC™ Blotting Standards, 250 µl
1610376
250 μl, pre-stained, Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, 75 kD), 50 applications
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(Discontinued) Precision Plus Protein™ Standard Plugs, unstained, Strep-tagged, recombinant
1610378
Pkg of 24, 1 mm thick agarose plugs containing ten Strep-tagged, recombinant proteins (10–250 kD), including three reference bands
Documents
Login Required
Number Description Options
4110025
Instruction Manual, Dual Color Precision Plus Protein™ Standards, Rev H
10018393
Instruction Manual, Precision Plus Protein™ Dual Xtra Standards, Rev D
4110182
Instruction Manual, Precision Plus Protein™ Kaleidoscope Standards, Rev G
4110024
Instruction Manual, All Blue Precision Plus Protein Standards, Rev D
3092
Precision Plus Protein Standards Flier, Rev B
6536
Precision Plus Protein™ Dual Color Standard Flier, Rev B
5957
Precision Plus Protein Dual Xtra Standards Product Information Sheet, Rev B
2800
Precision Plus Protein Dual Color Standards Competitive Card, Rev C
3093
Precision Plus Protein Standards Competitive Comparison Card, Rev B
3118
Protein Standards for Electrophoresis and Blotting Brochure, Rev D
2414
The Little Book of Standards, Rev D
2895
Protein Blotting Guide, Ver C
6040
Electrophoresis Guide, Interactive PDF, Rev C
6533
Introducing Bio-Rad’s TGX and TGX Stain-Free FastCast Acrylamide Kits, Rev A
3144
Using Precision Plus Protein Standards to Determine Molecular Weight, Rev B
3133
Molecular Weight Determination by SDS-PAGE, Rev B
5956
Precision Plus Protein Dual Xtra Standards — New Protein Standards with an Extended Range from 2 to 250 kD, Rev B
